Lecture 29 SRR F-2011

Lecture 29 SRR F-2011 - Biology 313, Lecture 29 Nov 2, 2011...

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Biology 313, Lecture 29 Nov 2, 2011 What is this? Hint: it’s killed 25 million people in the past 25 years
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Chapter 11 : Chromosome Structure Problems (through Ch. 11 ) Concept Checks (1-9) Worked Problems: 1 Comprehension and Application Questions at end of Chapter: 1-14; 21, 24-27; 29; 31-35
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Chapter 11 : Chromosome Structure and Transposable Elements 1. Tertiary DNA structure: chromosome packing 2. Centromeres and telomeres 3. Sequence variation in eukaryotes A. B. Types of DNA sequences C. Transposable elements
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C-value = amount of DNA in a cell C-value paradox : why is C-value so variable in eukaryotes? The amount of DNA does not correlate with biological complexity e.g., salamander and maize have 20 times more DNA than humans!!! Answer: Eukaryotes have “extra” DNA that does not code for a protein. What is this extra DNA?
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Denaturation (melting): process that separates the strands of double-stranded (ds) DNA when DNA is heated Renaturation (reannealing): process by which two complementary single-stranded (ss) DNA molecules pair Kinetics (To Elmo)
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Chapter 11 : Chromosome Structure and Transposable Elements 1. Tertiary DNA structure: chromosome packing 2. Centromeres and telomeres 3. Sequence variation in eukaryotes B. Types of DNA sequences C. Transposable elements
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Types of DNA Sequences in Eukaryotes Types are based on “complexity” from DNA renaturation kinetics 1. Unique sequence DNA 2. Moderately repetitive DNA 3. Highly repetitive DNA
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Types of DNA Sequences in Eukaryotes 1. Unique sequence DNA -Is transcribed, contains “ single copy genes and also gene families = similar but not identical copies of genes that arose through duplication of a single ancestral gene (several to a few hundred copies) - Members of gene families are usually isoforms of a given protein (they differ from one another in a few amino acids)
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Lecture 29 SRR F-2011 - Biology 313, Lecture 29 Nov 2, 2011...

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