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Human Fat as a Source of Stem Cells - Kacey Marra

Human Fat as a Source of Stem Cells - Kacey Marra - Human...

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Human Fat as a Source of Stem Cells Kacey G. Marra, Ph.D. Director, Plastic Surgery Research Laboratory Co-Director, Adipose Stem Cell Center Assistant Professor, Surgery and Bioengineering Faculty, McGowan Institute for Regenerative Medicine (MIRM) January 31, 2009
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What is Tissue Engineering? • The combination of biodegradable materials, cells and drugs to create new tissue
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Logical Sources of Autologous Cells • Blood • Bone Marrow • Adipose = Fat
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Our Lab has a Strong Focus on Fat “Pittsburgh Fat”
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Bone Marrow vs. Adipose Daniels, E.J., Scientific Data Series in Regenerative Cell Therapy, 2004, MacroPore Biosurgery.
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Largest tissue in the body (expendable) Autologous fat transplantation yields poor results: 40- 60% reduction in graft volume -Insufficient vascularization -Damaged adipocytes (~90% traumatized by mechanical forces of liposuction) -Remaining 10% tend to form cysts or localized necrosis postinjection Patrick, Anat. Rec., 2001, 263, 361 Adipose Tissue
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http://www.smh.com.au/articles/2003 /01/28/1043534062446.html http://www.michaeljfox.com/ How can we use FAT to treat diseases? http://www.centerforrestoration.com/ breastrecon/beforeafter-images.html www.smiletrain.org www.pittsburghsteelers.com
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What is our FAT source?
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http://www.plasticsurgery4u.com/procedure_folder/liposuction.html Liposuction Technique
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Abdominoplasty (Tummy Tuck) http://www.losangelestummytuck.com/tummy-tuck-pictures.cfm BEFORE AFTER
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Gastric Bypass Patients Pre-op 3 mo 6 mo 9 mo
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Post-Gastric Bypass Patients
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Isolation of Adipose-Derived Stem Cells [ASC] Receive Discarded Adipose Tissue
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Mincing, Digestion, Centrifugation of Adipose Tissue
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Cells have a flat, spindle-like structure similar to fibroblasts Isolation of Adult Stem Cells
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Zuk et al.
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