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BIO 113- SEA Lab Research Paper EMD

BIO 113- SEA Lab Research Paper EMD - Bacteriophages could...

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Introduction: Bacteriophages are most likely the most abundant organism in the world and can survive in any environment. A bacteriophage is a virus that infects bacteria. Bacteriophages are often called “phages” for short; phages are parasites specific to bacteria (NGRI, 2011). Bacteriophages are extremely specific for their host because in the first step of phage infection the phage attaches to a specific receptor on the bacterial cell surface [e.g. (Yemini et al., 2007)]. Phages cannot replicate or propagate outside their host bacteria (NGRI, 2011). They are ubiquitous and are not susceptible to antibiotics (NGRI, 2011). Once a phage gets attached to a bacterium, it inserts his DNA in the bacterium cell. Replication of the phage genome will then take place, until the host bacterium lyses (NGRI, 2011). Bacteriophages can be used in multiple ways. Scientists can use the genetic information to identify new genes that may be useful for different therapeutic applications (NGRI, 2011).
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Unformatted text preview: Bacteriophages could potentially be used to get rid of certain bacteria that cause different diseases. Felix dHerelle was one of the first to discover bacteriophages. In 1910 he saw clear spots (plaques) on a bacteria lawn, however scientist Edward Twort was credited for the independent discovery of phages at the same time. In our experiment we will discover, manipulate, and sequence a new phage. This phage will likely contain new genes and new gene arrangements, and the data that is collected will be used by other scientists to explore a variety of research areas (NGRI, 2011). References: Miri Yemini, Yaron Levi, Ezra Yagil, Judith Rishpon. 2007. Specific electrochemical phage sensing for Bacillus cereus and Mycobacterium smegmatis. Bioelectrochemistry. Volume 70(1): 180-184. NGRI Resource Guide, HHMI. 2011. Capture pp11-14....
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