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hwk - J W Fall'1 1 CH370 Name 1 Hackert HW 2(20 pts UTeID 1...

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Unformatted text preview: J W Fall '1 1 CH370 Name 1:: Hackert HW- 2 (20 pts) UTeID 1. You have visited your doctor about a "lump" on your back. She runs a genomics marker test using a DNA microarray to compare “normal" cells vs. "lump" cells. After 24 hours exposure. mRNA is harvested, cDNA prepared using red-dye markers for the “normal" ceti sample and green—dye markers for “your lump" cells. Any gene roduct that shows no difference in expression between the two cell tines would be indicated by a yet at to colored spot. (1) 2. Batance the following radioactive decay equation by filling in the blank with the missing item. at: o (1) a) “‘W e + r? “H 73 ti (1) b) A radioisotope has a rate constant of 0.037 f yr. Calculate the half-iife of the radioisotope. Half-life= { QM w Jslfi— ”Frat wfi .3“ QQQEQW e {8‘73“ (loft/«p (1) c) How many years will it take for a sampie of this radioisotope rated at 20 microCuries to undergo radioactive decay to the point where it loses 98% of its current activity ? (th years. ti: was“: ~o.t mime Mg?- : id: or r. byway 3. SDS gels are greatly improved in resotutton by running a “stacking” gel and a “resolving” or “running" get. a) Name two key property differences between the "stacking" get and the “resoiving” gel that contribute to the improved resoiution of running D SC PAGE. (1) a) {”5“ .. 1' .2516th p R in Liar/KC») refit ( (aim Q Gumbo O g as b) 0 3“. W” [Cr-W Zea a/Mfigflgmr M my cséficcc. 'W‘Jk 5812‘ What is the ro e of each of the following in performing SOS-PAGE? EUPH “ UM3 spilt .50 atxits'tflg in huge“ 3041:; grim N Quitting; "n.0,‘k0w31 in Farming L‘QE'L‘ (1) . -- . b) Coomassie Blue: m Wax SSGMA \‘i’ov VSLYQT‘Aa 4. The equation of motion for a small. spherical particle of mass (m) and frictional coefficient (f) that is initialiy at rest, and then acted on by a constant force (F) at time t = 0 is F —fv = ma. (From calculus recall that F - fv = m(dvldt) solves to v a (F/f) { 1 — exp(—ftlm)].) a) Show that such a particle will initialty accelerate but over time wilt approach a “maximat” velocity. (1) ”F? “W" . ‘9'"??? w a? VaxéC‘me‘q Art3>a5f~ssr>Jg «so w? \Imwu': (2) b) The diffusion constant for a protein is determined to be 0.258 x 10'6 cmgls at with T = 20° C. and t] = 0.01 (glcm-s). It has ai‘diameter'bf 80A, a density of 1.3 gicm3 and a vwbar of 0.73 cmalg protein. Calculate the frictionat coefficient ratio (flfmtn) for this protein and comment on the expected shape of the motecuie (spherical or not). M 0.0l I . ”‘3 a . "g wetsuits) 81% (to {0 cm”) 7.5 [o 3/. . ReT” , . 3 r,» . ... 2; t W» (3313.....1W3't‘ .,. z. s w . to 1% °~ E» . trio .2 i ‘} $0 71516;; til“: M , “it; . 5. What is typically measured by dynamic tight scattering (LS)? S) W} g: ““3 B ' Nfii'so (2) What wavelengths are normally employed in making circuiar dichroism (CD) spectra? Q - r . :3 «(Mtg-tit: RX 6. Determine the sedimentation coefficient (3) and molecular weight (M) for the sample that gave the following data when subjected to: A) a sedimentation velocity run using Schlieren optics. and B) a sedimentation equilibrium run using interference optics. Note: the figures below have been magnified to allow you to make measurements from the figures. The "r" can be determined from the reference points (r0) and the magnification factors. Assume T = 20° C, density of buffer = 0.9978 glmL, and v—bar = 0.737 cmaig for the protein, and n = 0.01 {gfcm-s) for both experiments. A) Sed. Vet. : co = 40,000 rpm, magnification factor (25):), r0 = 5.72 cm. (times are given in minutes). (4) Report “5" in proper units [ s = 3: v 19'“ ’3 5M. at 151$ ] (Show work and attach plot). (£40) {52) {65'} B) Sed Equilibrium: a) = 5200 rpm. magnification factor (25X), r0 = 6.75 cm. Caiculate M in g/mol (4pts) and (5) also estimate the concentration of the protein at the position with the white arrow (1 pt). Assume the cell path length to be 12.00 mm, l = 546 nm, and (dnfdc = 0.186 (t_:p"crt‘i3)‘1 . [Ma w i Q a ma 2 {gull ; [ janowa ’V i m. at (Show work and attach plot). I: t. "I . .‘I‘I "‘ a 7' .. ." '1”; , .‘.'_" “ _- - I. .i "r ‘ :- rr;':-._‘;"'I'IV:V: .. I: . l' '1'”- V- ‘ L ‘1- ‘ . _. '-.' . ‘ .. _ 1. ’1 . . ._ L; . _ ‘ ' _. u v . x. _ . ’ _ _ _ .. . . V I! . .' a: I' l 4 ' ‘. . - - I 7 _ ' o .5 _ - g. a “=_ _ ' , ' .s_ .. i . I ‘ I -‘ ." ... I .. ... - .-'_ . . .. . - . ' ..' '- I .‘f - ' . ' ‘- r ,' I .I _ is ' 3.. ' " "a ' . ' - . "‘- - i'. . , .. . . “ ‘ '; . ' ;_ '_ ._ ‘- " _ ' . 7 . _ .-. _. ., " _..‘ . ' * _ ‘ '. . . '. . '5. . ‘ I . - v a _ -. . - . x_. _ __ '. - - . . . ._ at“ ' -‘_"-_ .f ‘. I a .‘- . .-' "..l -' - -= :-' -"~ ‘5 oql __ ..‘_ - .» ."“' f 3, - . _ ' :27 - - H I. ‘ ~ V - I . . .. a 55.13% fi‘flim 7' ,4- _‘ ‘ _ i - . "'_".._-'~..t'-I ”‘21:"... a J'_,” l. n'__.___":‘__‘_if...- I : Iii ‘ .‘- l ”r I f .. ‘ a '. ax ~ ‘ .__ , sninpit 1'»;an V“ g Y“ 4‘“ We; a 0:,le 2:, M IV 1 WW 0 m; J 0v K 0W» " Nit: - v 9) w i .3...» I hereby declare that I did this assignment independently: Q U N" m WWQ Rfiée (”3“ firm“) maximally 5 m K ...
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