MBIlabreport - Hailey Holman Lab Section HE Lab Report:...

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Hailey Holman Lab Section HE Lab Report: Effect of UV light on Bacterial Growth Purpose: The presence of UV light has proven to damage the DNA of exposed microbes, leading to microbial death. In this experiment, we compared the time of exposure and the ability of the UV light to kill bacteria. The purpose was to observe the different rates at which UV light killed the bacteria on our agar plates. Methods and Results: We conducted our experiment using the Bacillus Subtilis culture. We began by labeling 6 agar plates; “control”, “30 seconds”, “1 minute”, “3 minutes”, “bibulous paper”, and “lid on.” We dipped a swab in the Bacillus Subtilis culture and rubbed the swab on the surface of each plate, making sure the surface was covered completely. We covered half of the “30 second”, “1 minute”, and “3 minute” plates with a piece of cardboard. We put each of the three plates in the UV light area and removed them after the amount of time labeled on each plate. We covered the last two plates (“bibulous” and “lid on”) with bibulous paper and a lid, respectively, and put them both in the UV light area. After 3 minutes, we removed them from the UV light. All six of the plates were then incubated at 37C. After removing the plates from the incubator, the control, bibulous
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This note was uploaded on 11/16/2011 for the course MICROBIOLO 131 taught by Professor Ferguson during the Spring '11 term at Miami University.

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MBIlabreport - Hailey Holman Lab Section HE Lab Report:...

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