HW 13-Chromatography

HW 13-Chromatography - HW
13‐Chromatography
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Unformatted text preview: HW
13‐Chromatography
 Due
Friday
Nov
11
 (1)  Substances
 A
 and
 B
 have
 retenBon
 Bmes
 of
 16.40
 and
 17.63
 min,
 respecBvely,
 on
 a
 30
 cm
 column.
 An
 unretained
species
passes
through
the
column
in
1.30
min.
The
peak
widths
(at
base)
for
A
and
B
are
1.11
 and
1.21
min.,
respecBvely.
Calculate
the
column
resoluBon,
the
average
number
of
plates
in
the
column,
 and
the
plate
height.
 (2)  List
the
variables
that
lead
to
zone
broadening
in
chromatography.
 (3)  Name
two
methods
for
improving
the
resoluBon
of
two
substances
on
a
column.
 ...
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