Enzyme Lab Report

Enzyme Lab Report - Effect of Inhibitors Temperature pH...

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Effect of Inhibitors, Temperature, pH, Substrate Concentration, and Enzyme Concentration on Acid Phosphatase Anthony M. Cavallaro Biology 107 Section 021 Tais Pimentel and Hao Zuo
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Anthony M. Cavallaro Effect of Inhibitors, Temperature, pH, Substrate Concentration and Enzyme Concentration on Acid Phosphatase Introduction Enzymes are essential in many processes in living organisms. Enzymes, usually proteins, catalyze reactions on the cellular level by lowering the activation energy required to trigger reactions and therefore expedite the reaction, making it more efficient. Enzymes are highly specific to the reactions that they catalyze. Enzymes have specific conformations and unique active sites, where it will bind with its substrate during the reaction. Upon completion of the reaction, the substrate is released from the active site and the enzyme can bind to another substrate to catalyze another reaction . Normally, uncatalyzed biological reactions tend to occur slowly. Enzymes solve this problem by creating an environment where reactions can occur more rapidly. Enzyme-catalyzed reactions are identifiable because they take place within the active site of an enzyme . Enzymes are important because of their unique conformations and the fact that they are not consumed by the reaction and can be used over and over again. Acid phosphatase, an enzyme that primarily originates from wheat germ, is commonly found in humans in small amounts. An increase in this enzyme can cause pathological conditions, such as prostatic cancer, liver disease, myocardial infarction, Paget’s disease and sickle cell anemia. Acid phosphatase, commonly referred to simply as ACP, can be detected by supplying an alternate substrate with which it can react. In this experiment, the substrate p- nitrophenyl phosphate was supplied. The result of the reaction of ACP and p-nitrophenyl phosphate forms p-nitrophenol, a yellow colored product whose intensity can be determined using spectrophotometry. The reaction that will take place in this experiment can be demonstrated by the following equation: p-nitrophenol phosphatase + H 2 O ACP p-nitrophenol + P i
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Anthony M. Cavallaro In this experiment, temperature, pH, inhibitors, enzyme concentration, and substrate concentration were studied to determine their effect on the amount of p-nitrophenol that acid phosphatase can produce. In the first test of this experiment, the activity of acid phosphatase should be reduced in
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This note was uploaded on 11/16/2011 for the course BIOL 107 taught by Professor Abbot during the Spring '08 term at UConn.

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Enzyme Lab Report - Effect of Inhibitors Temperature pH...

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