Photosynthesis - Anthony M. Cavallaro Biology 108 Section 4...

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Anthony M. Cavallaro Biology 108 Section 4 Photosynthesis I. Introduction Photosynthesis is the process by which plants convert energy from sunlight into a food source . Sunlight plays a much larger role than is initially evident. All of the foods we consume are products of the process of photosynthesis, the process that converts energy from the sun into chemical energy that can be utilized by plants and animals. Photosynthesis is performed primarily by green plants, protists, and bacteria. All these organisms convert carbon dioxide to carbohydrates in a cycle of reactions. Electrons for the reaction are obtained from water, which is then converted to oxygen. Energy for this process is provided by light, which is absorbed by pigments called chlorophylls and carotenoids. These pigments absorb different wavelengths of the spectrum of light. The pigments and the wavelengths they absorb make up the absorption spectrum. Chlorophyll absorbs red and blue light while carotenoids absorb blue-green light. Therefore, green and yellow light are not absorbed by these pigments in plants, making the plants green in appearance. Refer to Table 1 in the results section to see the different colors that each wavelength absorbs. In this experiment, we used spectrophotometry to observe the reaction of photosynthesis by comparing different samples consisting of chloroplast suspension, sodium phosphate buffer, water, and an alternate electron acceptor (DCPIP) from Spanacia oleracea . By varying the amount of DCPIP and chloroplast suspension in the samples, it allowed us to see the importance of the pigments and their role in the photosynthetic reaction. Next, we repeated the spectrophotometry exercise, creating four samples with the same concentrations of chloroplast suspension, phosphate
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Photosynthesis - Anthony M. Cavallaro Biology 108 Section 4...

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