Lecture 7 - PLB 113 - Lecture 7 II. Genome Organization and...

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PLB 113 - Lecture 7 II. Genome Organization and Gene Expression. F. Differential Gene Expression <= end of MT1 III. Gene Transfer and Epigenetics A. Gene Transfer Systems B. Agrobacterium tumefaiuns --------------------------------------------------------------------------------------------------------------------------- *complexity of mRNA shared with leaf + complexity of mRNA not shared with leaf = mRNA complexity of root. * all the different organs of the plants have about the same numbers of mRNA. Question of the Day • Each organ of the plant (except leaves and petals) possess mRNAs that are not present in leaves. • You have available leaf null mDNA and leaf, stem, root, and anther mRNA. • Design an experiment to show that anther RNAs not present in leaves are also not present in stems or roots. = show that some anther RNAs are specific to anther. Leaf Cell Stem Cell Anther (possibility 1) Anther (possibility 2) Non-shared are NOT Non-shared are unique = unique not present in other organs but anther Leaf null mDNA + stem mRNA = assume 5% of leaf null mDNA hybridizes * leaf null mDNA = mRNA that aren't present in leaf * 5% of the leaf null mDNA hybridized = % of mRNA that aren't present in leaf but present in stem. Leaf null mDNA + another mRNA = 5% of leaf null mDNA hybridized Leaf null mDNA (probe) + stem mRNA + anther mRNA = If it's possibility 1 => 5% of leaf null mDNA hybridized If it's possibility 2 => 10% of leaf null mDNA hybridized If non-shared RNA is unique, each set of RNA is going to hybridize with a fraction of leaf null mDNA Following conclusion after this type of experiment: ABC DEF ABC GHI ABC ABC GHI ABC MNO
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Comparison of mRNA Populations in Tobacco Organs - all the organs in the plant have about the same number of RNAs - common RNA = housekeeping genes and basic cellular functions - shared RNA = RNA shared between leaves and other organs. - organ-specific RNA = RNA that determine the shape and function of the organ. From this diagram we can see that all the RNA present in leaves and petal are shared. At the
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This note was uploaded on 11/16/2011 for the course PLB 40175 taught by Professor Amenta during the Spring '11 term at UC Davis.

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Lecture 7 - PLB 113 - Lecture 7 II. Genome Organization and...

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