104Lecture1

104Lecture1 - Lecture 1 3/19/11 Background reading in...

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Lecture 1 3/19/11 Background reading in textbook: 5 th edition: Chapter 1: pages 14-16 and pages 26-32 Chapter 8: pages 510-512 and pages 517-518 Chapter 9: pages 579-592 4 th edition: Chapter 1: pages 16-18 and pages 29-32 Chapter 8: pages 478-487 Chapter 9: pages 547-570 Lecture Outline: Introduction Procaryotes/Eucaryotes Examples of specialized cell types Tools: Microscopy Subcellular fractionation Immunochemical approaches
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Cell Biology – study of relationship of structure to function of cell Limit of eye to resolve is 200 µ m Cell Size Procaryotes: 0.5 – 2 µ m x 1 – 10 µ m (E. coli about 3 x 1 µ m) Eucaryotes: plant cell 10 – 200 µ m diameter animal cell 10 – 30 µ m diameter Organelles: Nuclei 3-8 µ m diameter Mitochondria and chloroplasts 0.5 µ m diameter Supramolecular assemblies: Ribosomes about 30 nm Membranes about 8 nm thick
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Key Historical Events: 1600’s – invention of compound microscope Hooke – pores in cork that he called cells Leeuwenhoek – saw protozoa and bacteria 1838 – Schleiden and Schwann proposed the cell theory that the cell is the simplest common unit of life Thus, study of cell biology has roots in morphology, which was the main approach used for over 200 years. This morphological approach was also used to establish the evolutionary relation- ships of organisms. We can now use genome analysis to determine these evolutionary relationships as shown by the “Tree of Life” on the next page. Now in the 21
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104Lecture1 - Lecture 1 3/19/11 Background reading in...

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