104Lecture7

104Lecture7 - Lecture 7 4/21/11 Background reading: MBOC 5...

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Unformatted text preview: Lecture 7 4/21/11 Background reading: MBOC 5 th Edition: Chapter 12: Pages 736 – 739 Chapter 13: Pages 749 – 753; 771-775; 779-787 4 th Edition: Chapter 12: pages 702 – 704 Chapter 13: Pages 731-737; 739-745 Outline: • Secretory system. • Green fluorescent protein (GFP) fusion proteins. • Golgi apparatus. • Processing of N-linked carbohydrate unit. • Retention of resident proteins of compartments. • Lysosomes. • Targeting of proteins to lysosomes. • Inclusion cell disease (I-cell disease). • Regulated secretion. • Constitutive secretory pathway. Asn-X-Ser Expanded view of the carbohydrate unit transferred onto the protein in the ER = N-acetylglucosamine (GlNAc) = Mannose = Glucose While in the ER, all three glucoses are trimmed off of the carbohydrate unit in a controlled process that assures that the protein is correctly folded before it can move onward in the secretory system. • Secretory System Route traveled by all proteins destined for following locations: Endoplasmic reticulum Golgi apparatus Lysosomes (or vacuoles) Endosomes Secretory vesicles Outside cell, including plasma membrane proteins Proteins first enter this system by transiting to lumen of ER....
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This note was uploaded on 11/16/2011 for the course BIS 40175 taught by Professor Amenta during the Spring '11 term at UC Davis.

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104Lecture7 - Lecture 7 4/21/11 Background reading: MBOC 5...

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