104Lecture10

104Lecture10 - Lecture 10 5/5/11 Background Reading: MBOC...

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Lecture 10 5/5/11 Background Reading: MBOC 5 th edition: Chapter 16: pages 965-991 MBOC 4 th edition: Chapter 16: pages 907-929 Outline: Vesicular transport- Molecular genetics approach. Mechanism of vesicular transport. Monomeric GTPases. Organelle biogenesis. Cytoskeleton – Overview. Intermediate filaments. Microtubules. Microtubule dynamics. Drug sensitivity of microtubules. Regulation of microtubules. Organelle movement.
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2) Vesicular transport Molecular Genetic approaches: Schekman was pioneer in these studies. Used yeast. Can easily grow, mutagenize and screen. ince secretory system so important, many mutants of it are lethal under ordinary conditions. Therefore used tempera- ture sensitive mutants. Cells grow normally at 25°C but lethal at 37°C. Using such mutants, Identified more than 25 genes. sing above biochemical and genetic approaches,identified many of the components involved in vesicular transport. Can divide into three separate processes: 1) Budding – vesicle formation 2) Docking – vesicle recognition 3) Fusion – fusion of vesicle with target compartment
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Budding: Involves accumulation of coat on cytosolic side of membrane. Identified different types of coats depending on what type of membrane is budding. Example: Clathrin – found in coats of vesicles mediating transport from the Golgi and the plasma membrane
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ocking: Involves specific recognition of vesicle for its target. Proteins involved are called snares. Postulated role of SNAREs in guiding vesicular transport
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Fusion: Involves specific proteins that must bring lipid bilayers very close to one another. Requires ATP.
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This note was uploaded on 11/16/2011 for the course BIS 40175 taught by Professor Amenta during the Spring '11 term at UC Davis.

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104Lecture10 - Lecture 10 5/5/11 Background Reading: MBOC...

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