Problem_Set_answers

Problem_Set_answers - Biology 52 Problem Set for Lectures 1...

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Biology 52: Problem Set for Lectures 1 and 2 1. Explain the differences between primary cell culture and the use of continuous cells lines. What are the advantages and disadvantages of using cultured cells as an experimental system? Ans: Primary cultures are derived directly from the animal and will only divide and grow for a discrete time period. Continuous cell lines, in contrast, are derived from transformed tissues (tumors) or embryonic tissues that retain their immortalized character and can be propagated indefinitely. Advantages are experimental ease of manipulation - you can add drugs to the culture medium, microinject the cells, and watch them using microscopy. All of these manipulations are technically more difficult with whole organisms. The drawback of their use is that cells in a dish are in a very artificial state and they lack the normal information that they would otherwise be receiving from other cells and extracellular matrix components in a tissue. 2. One of the central differences between eukaryotes and prokaryotes is that the latter lack internal membranous organelles. Why do eukaryotic cells have these membrane-bound compartments? Ans: Membrane-bound organelles are compartmentalized to allow specific biochemical reactions to take place efficiently. For example, the lysosome is an organelle that contains many proteases used to degrade food. Cells package these enzymes into this organelle so that they don't go around digesting the cell's own proteins in the cytoplasm.
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Problem_Set_answers - Biology 52 Problem Set for Lectures 1...

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