bio writeup

bio writeup - Kunal Bhan BISC 220: Friday Lab 11 1:50 pm...

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Kunal Bhan BISC 220: Friday Lab 11 – 1:50 pm TA: Erin Ragland Determining the Ideal Conditions at Which Amylase Best Functions Based on the Concentration and pH of Different Conditions I. Introduction All living things undergo various chemical reactions for the purpose of self-sustenance on a daily basis; while most of these reactions do take time, they are made faster and assisted by the presence of enzymes, biological catalysts which speed up the rate of the reaction without being consumed or modified. The activity and efficiency of these enzymes is also influenced by numerous factors, including temperature, pH, and enzyme concentration, which change both the shape and function of the enzyme; this would affect how substrates bind to the active site and thereby lead to influencing the products that would form or not form. Seeing how enzymes play a vital role in the cellular function of all living organisms, it would be important to study their function and the factors which affect them to better understand the influence they have. Studying the effects of concentration and pH on various enzymes is something which has been done many times in various experiments, looking at the effects in many different fields, including textile dyes (Cristavao, RO). In the experiment, there were two tests run to study the effect of both concentration and pH on the activity of an enzyme. In the first part of the experiment, five amylase solutions of differing concentrations were prepared and tested with an I 2 KI (potassium iodide) indicator to see how long it took for the starch to disappear, which then allowed for
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measuring how fast the reaction would be. In the second part of the experiment, 6 buffer solutions of differing pHs were prepared and tested again with I 2 KI indicator and run in the same format/conditions as the amylase solutions in the first part of the experiment, also to check for how pH would influence the rate of reaction. From the first experiment, the expected result was that as the concentration decreased, the reaction would rate would decrease, making it take longer for the starch to disappear. From the second experiment, the expected result was that as the pH became more neutral, it would take much less time for the disappearance of starch. II.
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bio writeup - Kunal Bhan BISC 220: Friday Lab 11 1:50 pm...

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