Bio 101 Laboratory 4

Bio 101 Laboratory 4 - Laboratory4:EnzymeActivity

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Laboratory 4: Enzyme Activity Enzyme  – an organic catalyst (usually a protein) that accelerates a specific chemical  reaction by lowering the activation energy required for that reaction Substrate  – a substance on which an enzyme acts Product  – a substance formed by a chemical reaction Enzyme for lab:  Alkaline Phosphatase Substrate for lab:  p-Nitro-Phenyl Phosphate (pNPP) Products for lab:  p-Nitrophenol + Inorganic Phosphate (pNP + P i ) Rate of Reaction:  ∆A/10min = (A f  – A i )/(t f  – t i ) × 10 min. A f  = final absorbance  A i  = initial absorbance t f  = final time t i  = initial time Enzyme Concentration  - the rate of an enzyme-catalyzed reaction would increase  proportionally with enzyme concentration, but then level off because there is a limited  amount of substrate Substrate Concentration  - the rate of an enzyme-catalyzed reaction would increase with 
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Bio 101 Laboratory 4 - Laboratory4:EnzymeActivity

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