ANAT 1503 Chapter 1 Outline - Chapter1...

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Chapter 1 Introduc)on to histology Histology means the science of 5ssue I . Basic Tissues A. Term 5ssue first used by Bichat, a French surgeon who said the body was made up of a variety of different 5ssues B. 4 types of 5ssue 1. Epithelial 2. Connec5ve 3. Muscular 4. Nervous II. Non‐cellular cons)tuents A. Intercellular matrix B. Body fluid‐ blood plasma III. Prepara)on of Histological Sec)ons A. Sec5ons‐ very thin slices of 5ssue B. Paraffin technique 1. Tissue sampling‐ obtained through a. biopsy b. surgical excision c. post mortem dissec5on * must be submersed in fixa5ve immediately aRer removal* 2. Fixing a. stops biological func5on of post mortem degenera5on. ‐ kills bacterial and other dx causing agents to lower risk of contamina5on when handled. 3. Dehydra5on a. increases alcohol because paraffin not water soluble and needs to be replaced with alcohol. Needs to be cleared with paraffin solvent that is miscible with alcohol. 4. Clearing
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a. Xylene –mixed with both alcohol and paraffin
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