ANAT 1503 Chapter 1 Outline

ANAT 1503 Chapter 1 Outline - Chapter

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Unformatted text preview: Chapter

1 Introduc)on
to
histology 



















Histology
means
the
science
of
5ssue I.
Basic
Tissues 















A.
Term
5ssue
first
used
by
Bichat,
a
French
surgeon
who
said
the
body
was
made
up
of
a
 






















variety
of
different
5ssues 















B.
4
types
of
5ssue 



















1.
Epithelial 



















2.
Connec5ve 



















3.
Muscular 



















4.
Nervous II.
Non‐cellular
cons)tuents 















A.
Intercellular
matrix 















B.
Body
fluid‐
blood
plasma III.
Prepara)on
of
Histological
Sec)ons 















A.
Sec5ons‐
very
thin
slices
of
5ssue 















B.
Paraffin
technique 





















1.
Tissue
sampling‐
obtained
through 




























a.
biopsy 




























b.
surgical
excision 




























c.
post
mortem
dissec5on 



























*
must
be
submersed
in
fixa5ve
immediately
aRer
removal* 





















2.
Fixing 






























a.
stops
biological
func5on
of
post
mortem
degenera5on.
 



































‐
kills
bacterial
and
other
dx
causing
agents
to
lower
risk
of
contamina5on
when
 





































handled.
 






















3.
Dehydra5on 
































a.
increases
alcohol
because
paraffin
not
water
soluble
and
needs
to
be
replaced
with
 



































alcohol.
Needs
to
be
cleared
with
paraffin
solvent
that
is
miscible
with
alcohol.
 






















4.
Clearing
 



























a.
Xylene
–mixed
with
both
alcohol
and
paraffin 























5.
Embedding 






























a.
warm
paraffin
wax
permeated
spaced
formerly
occupied
by
water.
 























6.
Sec5oning 





























a.
mounted
on
microtome
and
sliced
into
thin
shavings. 























7.
Staining
and
moun5ng 































a.
nuclear
region‐
hematoxin
(BASIC
STAIN) 






























b.
cytoplasm
region‐
Eosin
(ACIDIC
STAIN) IV.
Microscopes 











1.
Light
(op5cal)
microscope 











2.
Polarizing
microscope 











3.
Phase
contrast
microscope 











4.
Interference
microscope 











5.
Dark
field
microscope 



A.
Ultraviolet
microscope‐
allows
twice
resolu5on
of
ordinary
microscope 



B.
X‐ray
microscope‐
shorter
wave
length
and
higher
resolving
power 



C.
Electron
microscope=
used
high
velocity
electrons
as
a
light
source 













1.
Transmission
electron
microscope(TEM)
used
florescent
screen
(2‐D) 













2.
Scanning
electron
microscope
(SEM)
electrons
bombard
the
surface
of
specimen
and
deflects
 primary
and
emit
secondary
e‐
that
are
collected
by
special
elctor
screen
is
cathode
e‐
ray
tube.
 ...
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