Lab Write Up 3

Lab Write Up 3 - Elizabeth Pirinis March 23, 2010...

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Elizabeth Pirinis March 23, 2010 Antiseptics/Disinfectants and Bacteria Lab Write Up Background Information While antiseptics are used on people in removing bacteria from skin, disinfectants are used on surfaces and objects to have the same affect of removing bacteria that might be dangerous to people interacting with those surfaces. The most common types of bacteria that these are meant to remove are Ecoli and Bacillus Subtilis. Ecoli are a large group of bacterial germs that are typically rod-shaped, Gram negative and usually found in the lower intestine of humans and animals. They are known to contaminate foods and beverages and can survive several weeks on surfaces such as counter tops, and even a year in other materials such as compost (About E-Coli). They are versatile and well- adapted as they can survive temperature changes and harsh external conditions, making them harder to destroy. They are responsible for a significant amount of illness among humans that can lead to death if not identified and treated in a timely manner (Online Textbook of Bacteriology). Bacillus Subtilis are rod-shaped, Gram-positive bacteria that are usually found in soil and vegetation. The cell wall is a rigid structure outside the cell and is composed of peptidoglycan (consists of sugars and amino acids). They grow in a moderate temperature range (between 25-35 degrees Celsius). Through being used to existing in a stressful environment, they have developed a set of strategies that enables them to survive under relatively harsh conditions. The main thing that differentiates them from E-Coli is that 1
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they are non-pathogenic. They can contaminate food but rarely result in food poisoning, depending on the particular species. They can be used for agricultural seeds, such as vegetable and soybean seeds, as a fungicide, and are capable of producing toxins for insects and protecting crops (Microbe Wiki). There have been various experiments in the past that have been similar to the one that we conducted. In one in particular there were several bacteria that were tested against certain antiseptics and disinfectants. The antiseptics included sodium chloride, iodine, alcohol, and calcium hypochlorite. The disinfectants used were alcohol (up to 70%), synthetic detergents, phenolic compounds and sterilizing gases. As was done in our experiment the bacteria was tested against the power of the antiseptics and disinfectants. The results were generally that the disinfectants were more effective in killing the
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Lab Write Up 3 - Elizabeth Pirinis March 23, 2010...

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