FISH - streptavidin The streptavidin is located with...

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FISH No, not this sort, but " Fluorescent In Situ Hybridisation ". The technique of DNA-DNA hybridisation has been discussed in lecture 5 under the heading of Southern blotting . The ability of a single stranded DNA molecule to find and bind to its complementary strand is pretty amazing. In FISH it is exploited to the utmost. It is possible to visualise by hybridisation, the site of a fragment of DNA of as little as 1 - 2 kb (but more usually 40 - 50 kb) at an efficiency approaching 100%. The probe molecule is labelled with a hapten such as biotin. The biotin is located with
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Unformatted text preview: streptavidin. The streptavidin is located with antibodies. A fluorescent dye may be conjugated to the streptavidin and to the antibodies. When the spread chromosomes are illuminated by a UV lamp, a point of fluorescence can be seen where the probe / streptavidin / antibody / fluorescent dye multilayer sandwich has built up. From three to five layers of fluorescent antibodies are built up to amplify the signal....
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