Gene Cloning - Gene Cloning This is the general term given...

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Gene Cloning This is the general term given to the isolation of a specific fragment of target (e.g. human) DNA by propagating that fragment in a microorganism (usually a bacterium but sometimes a yeast). Using standard microbiological techniques, a single microorganism can be isolated and grown in culture carrying within its genome one small fragment of human DNA. The human DNA can readily be purified from the bacterium in gram quantities if necessary. For a more detailed explanation start here All clones come from libraries . Essentially these may be only one or other of cDNA or genomic , derived respectively from mRNA or genomic DNA, and it is vital to remember the distinction between them. Southern and Northern Blotting Before PCR and cheap fast sequencing changed our view of the universe that is genetics, the Southern Blot was a universal workhorse. There was not an experiment in molecular genetics which did not at some stage employ a Southern Blot. It is still a useful tool and you need to know about it so that you can interpret historical data. Named after its inventor, Prof. Ed. Southern, the blot is a fast way of analysing a
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This note was uploaded on 11/22/2011 for the course CHEMISTRY CHM1025 taught by Professor Laurachoudry during the Fall '10 term at Broward College.

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Gene Cloning - Gene Cloning This is the general term given...

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