Magnification - What microscopes do is to bring small...

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Magnification is the rati o of enlargement (or eduction) between the specimen and its image (either printed photograph or the virtual image seen through the eyepiece). To calculate magnification we multiply the power of each lens through which the light from the specimen passes, indicating that product as GGGX, where GGG is the product. For example: if the light passes through two,lenses (an objective lens and an ocular lens) we multiply the 10X ocular value by the value of the objective lens (say it is 4X): 10 X 4=40, or 40X magnification. Resolution is the ability to distinguish between two objects (or points). The closer the two objects are, the easier it is to distinguish recognize the distance between them.
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Unformatted text preview: What microscopes do is to bring small objects "closer" to the observer by increasing the magnification of the sample. Since the sample is the same distance from the viewer, a "virtual image" is formed as the light (or electron beam) passes through the magnifying lenses. Objects such as a human hair appear smooth (and feel smooth) when viewed with the unaided or naked) eye. However, put a hair under a microscope and it takes on a VERY different look! Working distance is the distance between the specimen and the magnifying lens. Depth of field is a measure of the amount of a specimen that can be in focus....
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This note was uploaded on 11/22/2011 for the course CHEMISTRY CHM1025 taught by Professor Laurachoudry during the Fall '10 term at Broward College.

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