geneticengineering

geneticengineering - • mRNA converted into cDNA •...

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Principles of genetic engineering L Mathias
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What is genetic engineering Genetic engineering, also known as recombinant DNA  technology , means altering the genes in a living organism to  produce a Genetically Modified Organism (GMO)  with a new  genotype.  Various kinds of genetic modification are possible: inserting a  foreign gene from one species into another, forming a  transgenic organism ; altering an existing gene so that its  product is changed; or changing gene expression so that it is  translated more often or not at all. 
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Basic steps in genetic engineering 1. Isolate the gene 2. Insert it in a host using a vector 3. Produce as many copies of the host as possible 4. Separate and purify the product of the gene
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Step 1: Isolating the gene
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Step 1: Alternative method (using reverse  transcriptase) Reverse transcriptase
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Unformatted text preview: • mRNA converted into cDNA • Complementary strand produced using DNA polymerase • Advantage – more mRNA in cell than DNA Step 2: Inserting gene into vector • Vector – molecule of DNA which is used to carry a foreign gene into a host cell Step 3: inserting vector into host • See worksheet Replica plating Step 4: Multiplication of the host cells by cloning • Large scale fermenters by cloning • All genetically identical because of asexual reproduction Step 5: Extraction of desired gene product This powerpoint was kindly donated to www.worldofteaching.com http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching....
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This note was uploaded on 11/23/2011 for the course BIO 100 taught by Professor Robinson during the Fall '08 term at BYU.

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geneticengineering - • mRNA converted into cDNA •...

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