NMR diagnosis


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Unformatted text preview: NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY -an ideal tool for Diagnosis & Drug Design B. HASEENA BANU Asst.Professor,SriPadmavathi School of Pharmacy,Tiruchanoor,Tirupati CONTENTS CONTENTS INTRODUCTION BASIC PRINCIPLE DIFFERENT TECHNIQUES APPLICATIONS OF NMR IN MEDICINE CLINICAL APPLICATIONS IN DIAGNOSIS DRUG DEVELOPMENT NMR Nobel prize laureates INTRODUCTION INTRODUCTION BASIC PRINCIPLE IN NMR Atomic nuclei – tiny magnets In external magnetic field ­ allign or oppose If irradiated with EMR of proper frequency – Resonance √ α βo PARAMETERS Chemical shifts Spin lattice relaxation time DIFFERENT TECHNIQUES DIFFERENT Based on nuclei H1 NMR C13 NMR F13 NMR & P31 NMR N15 NMR & O17 NMR CWNMR FTNMR 2D_NMR &3D_NMR COSY _CORRELATION SPECTROSCOPY MRI_MAGNETIC RESONANCE IMAGING ESR_ELECTRON SPIN RESONANCE SPECTROSCOPY APPLICATIONS OF NMR APPLICATIONS IN MEDICINE IN CLINICAL APPLICATION OF PROTON IMAGING IN DIAGNOSIS BRAIN Distinguishing gray matter & white matter Imaging posterior fossae, brain stem, spinal cord Detect demyelinating lesions, tumors, hemorrhages, infarctions ABDOMEN ABDOMEN o o o Metabolic liver disease Measures liver iron over load in hemochromatosis Focal areas of inflammation in chronic active hepatisis KIDNEYS o o Distinguishing renal cortex & medulla To evaluate transplanted kidney PELVIS o o Differentiates between Benign prostatic hyperplasia & prostatic carcinoma Detects bladder tumours MEDIASTINUM, HILUM, LUNG MEDIASTINUM, HILUM, LUNG o o Assessing abnormalities Respiratory gating HEART o o o Tomographic images of heart muscle, chambers, valvular structures ECHO­PLANAR TECHNIQUE Discrimination between infarcted, ischemic & normal myocardium BREAST o 3D­NMR & single­slice planar imaging in detecting breast abnormalities MUSCULO SKELETAL SYSTEM Demonstrates Osteo myelitis, tumor metastasis in vertebral bodies & pelvic bones Images of muscles, tendons, ligaments BLOOD VESSELS & FLOW Atherosclerotic vascular disease Assess blood flow in major vessels. INVIVO SPECTROSCOPY Chemical shift phenomenon Diagnosis of rare disease to inborn errors­ Mc Ardle’s syndrome INVIVO Analysis of Bone flouride content. PUTTING NMR IMAGING INTO PERSPECTIVE INTO Eliminates risk of x­radiation Excellent spatial & contrast resolution Detecting diseases at earlier stages NMR IN PHARMACEUTICAL RESEARCH RESEARCH Leading technology for 3­D structure determination of bio­macromolecules Studying protein structure SAR by NMR – Novel lead compounds Chemical shift mapping – Structural information on the binding modes and site positions Molecular dynamics, conformational analysis SHAPES NMR – SOLVE Structural genomics Structural genomics Identifying gene products in disease Targets of drug design. Development of Novel drug delivery systems Enhancing HTS assays Thousands of compounds Cryogenic NMR technology / cryoprobes NMR Nobel Prize Laureates NMR The Nobel Prize in Physics 1943, Ohostern, USA – Discovery of magnetic moment of proton The Nobel Prizein Physics 1952­ IsidorJ. Rafi,USA – Discovery of new methods for nuclear magnetic precision The Nobel Prize in Chemistry 1991 – Felix Bloch, USA and Edward M. Purcell, USA – Methodology of high resolution NMR. The Nobel Prize in Chemistry 2002 – Kurt Wuthrich, Switzerland – Determining 3­D Structure by NMR. The Nobel Prize in Medicine 2003 – Paul C. Lauterbur, USA and Peter Mans field, U.K. – Discoveries concerning MRI. THANKING YOU THANKING This powerpoint was kindly donated to www.worldofteaching.com http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching. ...
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This note was uploaded on 11/23/2011 for the course BIO 100 taught by Professor Robinson during the Fall '08 term at BYU.

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