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Exam-Two Review - MCB 2000 Study guide for EXAM TWO 1.State...

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MCB 2000 Study guide for EXAM TWO 1. State the difference between and give examples of horizontal and vertical genetic exchanges? 2. Name different RNA molecules. What is the role of each molecule? Codon vs. anticodon 3. Briefly describe replication, transcription, and translation. What is used as template for each process? 4. What is catabolic repression? Global repression? 5. Define operons,/their significance? Name two. Explain bacteria turn on/off an operon 6. What are the key players in protein synthesis? What is the role of each molecule? 7. What are the difference between DNA polymerase an RNA polymerase 8. What are the differences in synthesis of leading and lagging strands in DNA replication 9. What are the roles of helicase and ligase in DNA replication? 10. what is meant by (the significance of) DNA polymerase 3’-5’ exonuclease activity 11. What is meant by recombinant DNA? 12. Name the tools used to make a recombinant DNA. Example; how you cut DNA, how you introduce the DNA to the host cell, how to track the DNA. What is meant by gene library? 13. Define/Explain/describe: Complementary strands, Anti-parallel, Semiconservative replication, Double helix 14. What is the genetic material for viruses, DNA or RNA, single stranded or double stranded? 15. Where in eukaryotes and prokaryotes one can find DNA? 16. What are the products of: Replication, Transcription, Translation and Reverse Transcription? 17. What is the function of tRNA? 18. What are ribosomes made of? What is the difference between prokaryotes and eukaryotes ribosomes? What is the significance of this difference? 19.
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