Carlos Sandoval Matthew Drummer, Monday, Wednesday, 12 pm Post-Lab - Lab 3 Solution Concentration and Beer's Law July 8th, 2020 Purpose To prepare standard solutions of concentrations by dilution. As well as calculating the concentration of Albumin occurring in the experiments. Procedure Part 1 We begin by getting two UV cuvettes and adding 1 mL of BCG to 1mL and a few drops of albumin to each. Writing down our observations of color change. Next we get a multiwell tissue culture plate and add albumin in different ratios with excess BCG so that albumin is the limiting reactant in this experiment. Making it a 1:2 ratio. Then preparing six standard solutions of albumin in NaCl. Then taking the absorbance of each of the six solutions. Part 2 We get out the tissue culture plate and fill 6 adjacent wells with solution. That is determined by the given handout on how much to put in each well. From there we compare the colors of the solutions and estimate the concentration of albumin of each patient to 5 g/L.
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