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365s04syllabus

365s04syllabus - SYLLABUS for CHEMISTRY 365 BIOCHEMISTRY...

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SYLLABUS for CHEMISTRY 365 BIOCHEMISTRY Spring 2004 INSTRUCTOR Dr. Thomas M. Zamis I. Course Description A. Biochemistry (Prerequisites: Chem 248, 326, 328) Introduction to Biochemistry includes the chemistry of living cell components, and the nature and mechanism of cellular reactions. The chemical structures and biological functions of the most common small biomolecules, molecular assemblies and polymers of living organisms - including carbohydrates, proteins (enzymes), lipids, nucleic acids, coenzymes, and intermediate metabolites are covered. The reaction mechanisms, enzyme catalysts and regulation of the fundamental metabolic pathways are discussed - including glycogenesis, glycogenolysis, gluconeogenesis, glycolysis, the pentose phosphate pathway, fatty acid metabolism, the citric acid cycle, the electron transport system, oxidative phosphorylation, amino acid metabolism, the urea cycle and nucleotide metabolism. Biological information flow is outlined - including DNA replication and repair; RNA transcription, processing and control of gene expression; and protein biosynthesis. The focus of the laboratory portion of the course is on techniques for analysis of small biomolecule mixtures, biopolymer characterization, and enzyme isolation and kinetic analysis. Techniques include computer spreadsheet and word processing, uv-vis and colorimetric spectrophotometry, thin layer chromatography, gel permeation chromatography, gas- liquid chromatography, specific activity and kinetic assay, and electrophoresis. B. Information for Spring Semester TIMES: Lecture 1 T,R,F 8:00 - 8:50 Room Science A107 Lab 1 W 8:00 - 10:50 Science D118 Lab 2 T 14:00 - 16:50 Science D118 Lab 3 W 14:00 - 16:50 Science D118 REQUIRED MATERIALS TEXTBOOK: Garrett, Reginald; Grisham, Charles Principles of Biochemistry With a Human Focus ; Harcourt: Philadelphia, 2002. INSTRUCTOR: Dr. Tom Zamis Office B135 Phone 346-3258 e-mail: [email protected] Office Hours: T 9:00; W 11:00; R 9:00, 14:00 ; F 10:00 or other times by appointment .
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II. Course Outline Week # Dates Exam # Topics 1 1/20 - 1/23 Biomolecule Structures 2 1/27 - 1/30 Water: The Medium of Life 3 2/3 - 2/6 Bioenergetics 4 2/10 - 2/13 1 Amino Acids; Peptides; Proteins 5 2/17 - 2/20 Protein Biosynthesis; Enzymes 6 2/24 - 2/27 Enzymes 7 3/2 - 3/5 Carbohydrates 8 3/9 - 3/12 2 General Principles of Metabolism; Pathways 9 3/16 - 3/19 Carbohydrate Metabolism; Glycogen 10 3/30 - 4/2 Carbohydrate Metabolism; Glycolysis and Gluconeogenesis 11 4/6 - 4/9 Carbohydrate Metabolism; Pentose Phosphate Pathway 12 4/13 – 4/16 3 Lipids, Membranes, Transport; Fatty Acid Metabolism 13 4/20 – 4/23 Aerobic Metabolism – Citric Acid Cycle 14 4/27 - 4/30 Aerobic Metabolism – Electron Transport System 15 5/4 - 5/7 Nitrogen Metabolism: Amino Acid Degradation and Synthesis, Urea Cycle, Nucleotide Biosynthesis Exam 4 Tuesday May 11, 10:15 – 12:15
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III. Principal Assignments A. Textbook Reading Garrett, Reginald; Grisham, Charles Principles of Biochemistry With a Human Focus ; 2002.
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