Chapter12R

Chapter12R - BCH 4054 Fall 2000 Chapter 11 12 Review Lecture Notes Slide 1 Nucleic Acid Structure Linear polymer of nucleotides Phosphodiester

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Chapter 11 & 12 Review, page 1 BCH 4054 Fall 2000 Chapter 11 & 12 Review Lecture Notes Slide 1 Nucleic Acid Structure • Linear polymer of nucleotides • Phosphodiester linkage between 3’ and 5’ positions • See Figure 11.17 Slide 2 Nucleic Acid Sequence Abbreviations • Sequence normally written in 5’-3’ direction, for example: N N N N H2N O H O H H H H P O O- O H N N N O NH2 N O H H H H H O P O O- O N H2N O N O H O H H H H P O O- O H N O O N O H O H H H H P O O- O O Adenine Guan ine Cy tosine Thymine Slide 3 Sequence Abbreviations, con’t. Let Letter stand for base: A G C T P P P P P 5'-end 3'end Let Letter stand for nucleoside pApGp CpTp 5'-end 3'-end Let Letter stand for nucleotide AGCT 5'-end 3'-end
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Chapter 11 & 12 Review, page 2 Slide 4 Biological Roles of Nucleic Acids • DNA carries genetic information • 1 copy (haploid) or 2 copies (diploid) per cell • See “History of Search for Genetic Material” • RNA at least four types and functions • messenger RNA—structural gene information • transfer RNA—translation “dictionary” • ribosomal RNA—translation “factory” • small nuclear RNA—RNA processing Slide 5 DNA Structure • Watson-Crick Double Helix • Clues from Chargaff’s Rules • A=T, C=G, purines=pyrimidines • Helical dimensions from Franklin and Wilkins X-ray diffraction studies • Recognition of complementary base pairing possibility given correct tautomeric structure (See Fig’s. 11.6, 11.7, 11.20) Slide 6 Nature of DNA Helix • Antiparallel strands • Ribose phosphate chain on outside • Bases stacked in middle like stairs in a spiral staircase • Figure 11.19—schematic representation • Complementary strands provide possible mechanism for replication • Figure 11.21 representation of replication process
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Chapter 11 & 12 Review, page 3 Slide 7 Size of DNA Molecules • 2 nm diameter, about 0.35 nm per base pair in length • Very long, millions of base pairs Organism Base Pairs MW Length SV 40 virus 5.1 Kb 3.4x10 6 1.7 μ m λ phage 48 Kb 32 x 10 6 17 μ m E. coli 4,600 Kb 2.7 x 10 9 1.6 mm Yeast 13,500 Kb 9 x 10 9 4.6 mm Human 2.9 x 10 6 Kb 1.9 x 10 12 0.99 m Slide 8 Packaging of DNA
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This note was uploaded on 11/27/2011 for the course BSC 5936 taught by Professor Staff during the Spring '08 term at FSU.

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Chapter12R - BCH 4054 Fall 2000 Chapter 11 12 Review Lecture Notes Slide 1 Nucleic Acid Structure Linear polymer of nucleotides Phosphodiester

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