invertasechartcalcs

invertasechartcalcs - solution moles sucrose/minute = mL...

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Examples from crude extract on Purification Table. mg protein 25.3 μ moles sucrose/minute = mL enzyme solution 1.12 mg protein 28.3 mL enzyme solution μ moles sucrose/minute = = SA mg protein μ moles sucrose/minute mL enzyme solution 28.3 1.12 = - mg protein 0.10 mL 4.10 mL 18.0 mL/mg 0.000 0.490 mL enzyme solution μ moles sucrose/minute = 0.10 mL 1 1 μ moles 3 min 1 0.0191 L - 1330 M -1 (-0.012) 0.580 [mg protein/mL solution] [moles sucrose/Liter] mL enzyme
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Unformatted text preview: solution moles sucrose/minute = mL enzyme solution 1 10-6 mole 1 moles minutes 1 Liters final solution-slope intercept SPECIFIC ACTIVITY =-mg/mL 0.10 mL 4.10 mL slope intercept BRADFORD ASSAY SPECIFIC ACTIVITY CALCULATIONS FOR INVERTASE LAB X X X X X X X X X ABS X ABS ENZYME UNITS ASSAY (diluted sample) (diluted sample) (diluted sample) Chem 365 10-6 mole...
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