michaelismentenanalysis

michaelismentenanalysis - 5.00 6.00 7.00 8.00 9.00 0.000...

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Kinetics of Enzymes Following the Michaelis-Menten Model Determination of Parameters K m and v max Michealis-Menten Mechanism E + S <=> ES <=> EP <=> E + P Michealis-Menten Equation v 0 /v max = [S] 0 (K M + [S] 0 ) Lineweaver-Burk Equation 1/v 0 = (K M /v max )(1/[S] 0 ) + 1/v max Eadie-Hofstee Equation v 0 = -K M (v o /[S] o ) + v max Hanes-Woolf Equation [S] 0 /v 0 = (1/v max )([S] 0 ) + K M /v max Table 1. Data from Michaelis-Menten Kinetic Experiments on Enzyme [S] 0 (mM) v 0 ( μ moles/min) 1/[S] 0 1/v 0 v 0 /[S] 0 [S] 0 /v 0 0.00 0.000 2.70 0.123 0.370 8.13 0.0456 22.0 5.40 0.179 0.185 5.59 0.0331 30.2 8.09 0.210 0.124 4.76 0.0260 38.5 10.80 0.226 0.093 4.42 0.0209 47.8 40.00 0.300 Figure 2. Lineweaver-Burk Plot of Kinetic Experiments on Enzyme y = 13.466x + 3.1278 0.00 1.00 2.00 3.00 4.00
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Unformatted text preview: 5.00 6.00 7.00 8.00 9.00 0.000 0.100 0.200 0.300 0.400 1/[S] Figure 3. Eadie-Hofstee Plot of Kinetic Experiments on Enzyme y = -4.241x + 0.3177 0.000 0.050 0.100 0.150 0.200 0.250 0.0000 0.0100 0.0200 0.0300 0.0400 0.0500 v/[S] Figure 4. Hanes-Woolf Plot of Kinetic Experiments on Enzyme y = 3.1815x + 13.141 0.0 10.0 20.0 30.0 40.0 50.0 60.0 0.00 5.00 10.00 15.00 [S] Figure 1. Plot of Initial Reaction Velocity vs [Substrate] for Michaelis-Menten Model 0.000 0.050 0.100 0.150 0.200 0.250 0.300 0.350 0.00 10.00 20.00 30.00 40.00 50.00 [S] (mM)...
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This note was uploaded on 11/27/2011 for the course CHM 365 taught by Professor Thomaszamis during the Spring '06 term at FSU.

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