exam_1 (1) - DOWNSTREAM PROCESSING Exam#1 Open Book and...

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D OWNSTREAM P ROCESSING Exam #1 Open Book and Open Notes Problem 1 (Cell Disruption) The data given from a particle size analysis (NICOMP model 270) of yeast cells before and after one pass through a Gaulin-type high-pressure homongenizer at 8,000 psi indicate a mean particle size before homogenization of 5.2 micron and a mean particle size after homogenization of 3.5 micron. After one pass through the homogenizer, only 28% of the cellular protein is released. a) How many passes are required to achieve 90% release of cellular protein? b) Concerned about degradation of your desired protein product, you decide to attempt to achieve a high level of recovery by increasing pressure rather than number of passes. Your equipment will allow you to operate at a maximum pressure of 10,000 psi, so this is the pressure at which you conduct further experiments. At 10,000 psi and a single pass through the homogenizer, you are disappointed to find that you can release only 40% of the cellular protein. What is the pressure dependence of the disruption rate constant?
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exam_1 (1) - DOWNSTREAM PROCESSING Exam#1 Open Book and...

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