lec14_04042007_w - 10.37 Chemical and Biological Reaction...

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10.37 Chemical and Biological Reaction Engineering, Spring 2007 Prof. K. Dane Wittrup Lecture 14: Kinetics of Non-Covalent Biomolecular Interactions This lecture covers : Significance, typical values and diffusion limit, approach to equilibriu, and multivalency Noncovalent Interactions Protein Ligand Complex P + L C k k on off U Figure 1. Protein-ligand binding. Association rate = kC on p C L Dissociation rate = off c @ equilibrium, = on p C L off c 1 s CC pL k == o f f K Ck d co n L mol s In general, for protein-protein interactions, km 51 1 on 10 o l s half-time for complex dissociation ln 2 τ 12 = k off K d types mM milliseconds non-specific stickiness μ M (micromolar) milliseconds-seconds cell surface, multi valent nM minutes-hours antibodies, enzymes pM hours-weeks growth factors fM (femtomolar) weeks-months hycholase inhibitors stronger interactions Fractional saturation Y cc po , c + C p Cite as: K. Dane Wittrup, course materials for 10.37 Chemical and Biological Reaction Engineering, Spring 2007. MIT OpenCourseWare (http://ocw.mit.edu), Massachusetts Institute of Technology. Downloaded on
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This note was uploaded on 11/27/2011 for the course CHEMICAL E 10.302 taught by Professor Clarkcolton during the Fall '04 term at MIT.

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lec14_04042007_w - 10.37 Chemical and Biological Reaction...

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