Midterm2 - 10.37 Exam 2 25 April 2007 100 points Problem 1 35 points A protein and ligand bind reversibly with Kd = 10 nM The association rate

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10.37 Exam 2 25 April, 2007 100 points Problem 1: 35 points A protein and ligand bind reversibly with K d = 10 nM . The association rate constant k on = 2x10 4 M -1 s -1 . The two species are mixed at an initial protein concentration of 3 nM and an initial ligand concentration of 0.2 nM. a) At equilibrium, what fraction of the ligand will be complexed with protein? (15 points) b) At what time will the fraction of ligand in complex reach 95% of the equilibrium value? (20 points) Justify any assumptions you make to simplify equations. Problem 2: 30 points A surface-catalyzed reaction follows Rideal-Eley kinetics as follows: A A k k A SA ⎯⎯⎯→ + ←⎯⎯⎯ S 1 2 k A A S +⎯ →+ Where A and A 2 are in the gas phase, S is a reactive site on the surface, and AS is a molecule of A adsorbed to a reactive site. Assuming that: adsorption of A is at rapid equilibrium reaction of AS with A is rate-limiting desorption of A 2 is very rapid Derive the steady-state rate law for production of A 2 as a function of the concentration of
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This note was uploaded on 11/27/2011 for the course CHEMICAL E 10.302 taught by Professor Clarkcolton during the Fall '04 term at MIT.

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Midterm2 - 10.37 Exam 2 25 April 2007 100 points Problem 1 35 points A protein and ligand bind reversibly with Kd = 10 nM The association rate

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