exam_2 (1) - DOWNSTREAM PROCESSING Exam #2 Open Book and...

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D OWNSTREAM P ROCESSING Exam #2 Open Book and Open Notes Problem 1 (Membrane Filtration) Your goal is to concentrate a recombinant therapeutic protein, HR-I, that is eluted from an anion exchange column at 1.5 g-protein/L to a final concentration of 20 g/L. The concentration is done by tangential flow filtration across a 30,000 MW cut-off membrane in which the retentate is recirculated across the membrane from a reservoir. In laboratory experiments using a 100 cm 2 membrane the filtrate flux is 100 L/m 2 -hr at the initial concentration and cannot be increased by raising the pressure above 5 bar when the flow rate is 1 m/s. Also, the available literature data show that when the bulk protein concentration is continually increased under the same operating conditions, the flux becomes zero when the concentration of HR-I reaches 200 g/L. In the pilot scale process being developed for eventual scale-up to manufacturing, you have available a 1 m 2 membrane system that can be operated at the same pressure and flow rate as the
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exam_2 (1) - DOWNSTREAM PROCESSING Exam #2 Open Book and...

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