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lec4_igfi_trans - Diagram of IGF-I Ribbon Structure removed...

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Diagram of IGF-I R ibbon S tructure removed due to copyright considerations. See Figure 1 in Vajdos, F. F., et . al. "Crystal Structure of Human Insulin-like Growth Factor-1: Detergent Binding Inhibits Binding Protein Interactions." Biochemistry 40, no. 37 (2001).
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Figure by MIT OCW. COOH COO + H + ( ) D V I G T T P Q P A E E D M G C V F Q L A D V L E A G C N-terminus IGF-1 Analog C-terminus L T G Y C A P L P A C C F S S S S G G Y C D L A 70 6 missing 48 47 52 18 61 S L N F F Y + R + R + R + R + K + R + K + K + R D = Asp E = Glu pK ~ 4 pK ~ 10.5 -12.5 K = Lys R = Arg NH 3 + NH 2 + H + ( ) + (9) (9) (-) (+)
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0 0.5 1 1.5 2 0 400 800 1200 1600 2000 TIME, minutes Downstream Conc./ Upstream Conc., % τ lag = 267min <1nM I-IGF-I added upstream 100nM IGF-I 100nM IGF-I 125
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0 0 20 40 60 80 100 FRACTION NUMBER ACTIVITY, CPM B IGF F 3 × 10 6 2 × 10 6 10 6
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Binding of Insulin-Like Growth Factor-1 (IGF-1) to IGF Binding Proteins in Extracellular Matrix (NOT CELL SURFACE RECEPTORS) Figure by MIT OCW. After Figure 2A in Bhakta, N. R., et . al. "The Insulin-like Growth Factors (IGFs) I and II Bind to Articular Cartilage via the IGF-binding Proteins." J Biol Chem 275, no. 8 (2000).
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