lecture8 - 10.492 - Integrated Chemical Engineering (ICE)...

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10.492 - Integrated Chemical Engineering (ICE) Topics: Biocatalysis MIT Chemical Engineering Department Instructor: Professor Kristala Prather Fall 2004 Lecture # 12, 13, 14 – Putting It All Together: Process Design for Bioconversions Handout: Process Design Guidelines This lecture is intended as a review of the material we’ve covered in class to-date, with the objective being to understand a methodology for overall process design for bioconversions. We have not, and will not now, address any isolation process design. The one note to make here is that if you are designing the reaction phase, you should do so in consultation with those responsible for the isolation steps, so that you will not inadvertently design a process to make a product that can’t be easily isolated! 1. The Basics There are three questions that need to be answered for any process design: (1) How much? (2) How long? (3) How large? How much… material do you need to supply to your customer, 100 g, 1 kg, 10 kg? product can you obtain per unit of reaction volume (substrate/product solubility)? How long does one batch take to run in your process per unit of reaction volume? do you have to make your delivery? How large… is(are) the reaction vessel(s) that you have at your disposal for the reaction? of a vessel can you practically and safely operate? The answers to these questions determine the nature of your process and the configuration of the final design. Your ultimate goal is to deliver at least the requested amount of material on time using the resources you have available. Let’s look back at our design development process to see how we address these issues. 2. A Design Strategy Your basic strategy will be as follows, for both purified enzymes and whole cells: 1. Select a catalyst 2. Establish a baseline process 3. Optimize the process 4. Determine how to run the process to meet a target delivery Dr. Kristala L. Jones Prather, Copyright 2004. MIT Department of Chemical Engineering
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Lecture #8, p.2 (Handout provided with the following table) Let’s look in a little more detail at each of these steps: 1. Select a catalyst (screening) Enzyme Process Whole Cell Process ~10-20 enzymes, based on commercial availability Set-up identical reactions, keep enzyme loading (mass) constant Choose enzyme with good conversion, high selectivity ~100-200 strains for pre-existing library, ~10X more for environmental library Add same amount of substrate, normalize to cell mass Choose cell with good specific activity, high selectivity, (if possible) low by-products Remember that the number of catalysts you have to screen with whole cells will
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This note was uploaded on 11/27/2011 for the course CHEMICAL E 20.410j taught by Professor Rogerd.kamm during the Spring '03 term at MIT.

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lecture8 - 10.492 - Integrated Chemical Engineering (ICE)...

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