# ps_3 - DOWNSTREAM PROCESSING Problem Set#3 Problem 1 You...

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D OWNSTREAM P ROCESSING Problem Set #3 Problem 1 You are working with a chromatography column that has a 30 cm diameter and 40 cm length, is packed with 10 µ m particles ( ε = 0.4), and has a maximum allowable pressure drop of 70 bar. A protein of interest is to be eluted from the column using a mobile phase with a density ( ρ ) of 1000 kg/m 3 and a viscosity ( µ ) of 0.002 Pa-sec. a) Calculate the fluid velocity in the column if the measured pressure drop is 50 bar. b) A mixture with a viscosity of 0.05 Pa-sec is injected in the column and eluted at the fluid velocity calculated above. Estimate the maximum volume of the mixture that can be safely fed. ( Hint: consider the volume of injected sample as a plug that travels through the column. ) Problem 2 Immunoglobulin G (IgG) is to be purified from a mixture of proteins. At lab scale, a gel filtration chromatographic column with 1.6 cm diameter and 40 cm length is used with an elution flow rate of 1 cm 3 /min. The volume of feed injected was optimized to 4 cm

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ps_3 - DOWNSTREAM PROCESSING Problem Set#3 Problem 1 You...

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