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1548839640-quiz_1_key - Mic102/Win08/Appleman Quiz 1 23...

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Mic102/Win08/Appleman Quiz 1, 23 January 2008 This quiz is to represent only your own work . As a UC Davis student, you are bound by an honor code; if you can’t be trusted with that, can you be trusted in a career in, say, the health professions? This quiz is closed book, closed note. Please hand in a blue scantron with the answers to the multiple choice section of the quiz. Make sure that it has your name, student I.D.#, and “ Test form C ” filled in. You will also need to hand in the last two pages of this quiz, with your name on each. QuickTimeª and a TIFF (LZW) decompressor are needed to see this picture. Here’s a figure from a paper describing the discovery and characterization of an interesting prokaryotic organism. The scale bar in B and C is 1 μ m.
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The following questions refer to the picture on the front of the quiz form. 1. Figure D shows the growth of this organism over a range of temperatures. What is its optimal growth temperature? a) 85 ° C b) 107 ° C—generation time is shortest at this temperature c) 120 ° C d) >120 ° C 2. From the data in figure D, this organism is a a) hyperthermophile—growth rate is maximal over 100 ° b) mesophile c) halophile d) psychrophile 3. From the data in figure D, what probably happens to this organism at a temperature of 22 ° C (room temperature) a) its cell wall ruptures b) its proteins denature c) its cell membrane is no longer fluid in two dimensions d) its DNA becomes fragmented 4. From the data shown, one adaptation this organism likely has to suit its growing conditions is a) a thick slime layer b) a tendency to grow in biofilms c) gliding motility d) a cytpolasmic membrane made of a monolayer rather than a bilayer 5. The cells pictured in B and C were grown in the laboratory in a medium that contained no sugars, no amino acids, a little NH3, and trace amounts of minerals. This is an example of a) a rich medium b) a defined rich medium c) a defined minimal medium d) none of the above 6. This organism was grown using a medium that contained formate (HCOO-) and CO2 as the only forms of carbon; no oxygen was present. Panel A indicates that it was converting Fe +++ into Fe ++ . From this a) we can conclude the organism makes energy by fermentation b) we can conclude the organism makes energy by respiration—it is oxidizing formate and reducing Fe; just because no oxygen is available doesn’t mean its fermenting, and anyway, fermentation is impossible with formate as a start.
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