49705523-old_quiz_3_key

49705523-old_quiz_3_key - Mic102/Fa07/Appleman Quiz 3, 21...

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Mic102/Fa07/Appleman Quiz 3, 21 November 2007 This quiz is to represent only your own work . As a UC Davis student, you are bound by an honor code; if you can’t be trusted with that, can you be trusted in a career in, say, the health professions? This quiz is closed book, closed note. Please hand in a blue scantron with the answers to the multiple choice section of the quiz. Make sure that it has your name, student I.D.#, and “ Test form A ” filled in. You will also need to hand in the last page of this quiz, with your name on it. Recently, the entire genome of Vibrio fischeri was sequenced. As we know, we can infer a lot of interesting stuff from a genome sequence. QuickTimeª and a TIFF (LZW) decompressor are needed to see this picture. QuickTimeª and a TIFF (LZW) decompressor are needed to see this picture. TIFF (LZW) decompressor are needed to see this picture.
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1. The genome of Vibrio fischeri is unusual for a bacterium in that it includes two chromosomes and one plasmid. This means that a) its genome is unusually large b) it has two circular pieces of DNA with essential genes c) it has three circular pieces of DNA with essential genes d) it has two circular pieces of DNA with positive supercoiling and one with negative supercoiling 2. The Vibrio fischeri genome is 4.2 million bases in size. Approximately how many genes does it have? a) 4.2 million b) 420,000 c) 4,200 d) can’t tell 3. The DNA in the Vibrio fischeri genome is methylated at specific adenine bases. Why? a) so that the DNA is not digested by a Vibrio fischeri restriction endonuclease b) it is a signal for transcription initiation c) untranscribed regions are methylated d) it increases supercoiling 4. The genes in Vibrio fischeri are organized in operons. These are a) global regulatory proteins b) DNA domains c) consensus binding sites for repressor proteins d) groups of genes transcribed from a single promoter 5. An examination of the Vibrio fischeri genome reveals many examples of inverted repeats immediately upstream of promoters. These are a) binding sites for activator proteins b) binding sites for repressors c) binding sites for RNA Polymerase d) terminators 6. Vibrio fischeri lives in a changing environment, subject to fluctuations in temperature, salinity, &c. It can survive these thanks to global regulatory systems, which a) change promoter sequences in response to environmental conditions b) change the expression individual genes within an operon c) cause the cell to swim away from danger d) change the expression of many operons throughout the genome in a coordinated manner 7. One of the best-studied global responses is the heat-shock response, which is mediated
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49705523-old_quiz_3_key - Mic102/Fa07/Appleman Quiz 3, 21...

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