Ch_1_Preview_of_Cell - Brightfield b Fluorescence c Phase...

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Preview of the Cell Biol-003 Spring 2010 1 A. Cell Theory 1. Schleiden (1838)—all plant tissues composed of cells 2. Schwann (1839)—all animal tissues composed of cells a. All organisms consist of one or more cells b. Cell = basic unit of structure in all organisms 3. Virchow (1855)—all cells arise from pre-existing cells a. These discoveries made possible by microscopic examination of living tissue B. Microscopes 1. Light microscope capable of total magnification of 40-1000X (1-100 m) 2. Electron microscope required to view objects at nm level a. Resolution = minimum distance between two points b. Resolving power = ability of microscope to distinguish adjacent items as separate points 3. Different types of light microscopes a.
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Unformatted text preview: Brightfield b. Fluorescence c. Phase contrast d. Differential interference e. Confocal 4. Electron microscopes a. Transmission b. Scanning 5. X-ray diffraction C. Emergence of Modern Cell Biology 1. Modern cell biology combines three areas of study a. Cytology—study of cell structure o Began with development of first microscopes o Continuing with more modern techniques of microscopy b. Biochemistry—chemical structure and techniques for studying cell functions o Ultracentrifugation o Chromatography o Electrophoresis o Radioactive isotope labeling c. Genetics—study of inheritance o Mendel’s laws, based on first scientific studies o DNA as genetic material o Genome maps o Cloning o Genetic engineering D. Process of Science...
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