CH8-2 - Prokaryote vs Eukaryote mRNA Prokaryote Eukaryote...

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Prokaryote vs. Eukaryote mRNA Prokaryote Eukaryote transcription Gene (DNA) Primary transcript (RNA) translation transcription RNA processing Mature mRNA translation polypeptide
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Brainstorm What are examples of mRNA processing in eukaryotes? Answers: 5’ methylated cap 3’ Poly A Tail RNA Splicing remove introns Alternative splicing
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___5 ’ Methylated Cap __ Step 1: “Backward” G is added by a special ___capping enzyme__ via a triphosphate bridge Step 2: _methyl transferase___ adds methyls to G and 1+ succeeding nucleotides Fig. 8.13
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______3 ’ Poly-A Tail___________ Fig. 8.14 Step 1: ___Ribonuclease__ cuts mRNA 11-30 nucleotides downstream of AAUAAA (recognition element) Step 2: __Poly A polymerase___ adds multiple A’s onto 3’ end Step 1 Step 2
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5’ cap Translation initiation factor A Poly-A binding protein
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Exons – sequences found in a gene’s DNA and mature mRNA (ex pressed regions)
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This note was uploaded on 11/27/2011 for the course BIOL 12 taught by Professor Kim during the Spring '10 term at LSU.

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CH8-2 - Prokaryote vs Eukaryote mRNA Prokaryote Eukaryote...

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