CH9-1 - Chapter 9, Part I DNA at High Resolution;...

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Chapter 9, Part I DNA at High Resolution; Recombinant DNA technology (i.e. biotechnology) Tools used in recombinant DNA technology Restriction Enzymes Biological function Role in recombinant DNA technology Gel Electrophoresis Restriction Maps
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Restriction Enzymes Naturally found in bacteria Defend against viruses “Chew up” viral DNA How is the bacterium’s own DNA protected? BUT, they can cut any non- methylated double stranded DNA Me
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Racecar Rats live on no evil star Go hang a salami, I’m a lasagna hog
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Restriction Enzymes -TCGA- -AGCT-
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Cutting frequency depends on length of recognition sequence
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Gel electrophoresis Why is DNA charged?
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Gel electrophoresis Preparing an agarose gel for electrophoresis
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Loading DNA fragments onto an agarose gel and performing electrophoresis Gel electrophoresis separates DNA fragments according to size
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Visualizing the DNA fragments on the agarose gel Gel electrophoresis separates DNA fragments according to size How can we see DNA?
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This note was uploaded on 11/27/2011 for the course BIOL 12 taught by Professor Kim during the Spring '10 term at LSU.

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CH9-1 - Chapter 9, Part I DNA at High Resolution;...

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