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C G C A A A PROBE MOLECULES
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A C TOMO NARASHIMA PROBE MOLECULES are used to locate DNA strands encoding paths that pass through the intermediate cities (Boston and Chicago). Probe molecules containing the complementary DNA name of Boston (AGCCTGAC) are attached to an iron ball suspended in liquid. Because of Watson-Crick afﬁnity, the probes capture DNA strands that contain Boston’s name (TCGGACTG). Strands missing Boston’s name are then discarded. The process is repeated with probe molecules encoding the complementary DNA name of Chicago. When all the computational steps are completed, the strands left will be those that encode the solution GCAGTCGGACTGGGCTATGTCCGA. Atlanta) and the Watson-Crick complement of the ﬁrst name of the end city (GGCT for Detroit). These two primers worked in concert: the ﬁrst alerted DNA polymerase to copy complements of sequences that had the right start city, and the second initiated the duplication of molecules that encoded the correct end city. PCR proceeds through thermocycling, repeatedly raising and lowering the temperature of the mixture in the test...
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This note was uploaded on 11/28/2011 for the course COMP 790 taught by Professor Staff during the Fall '08 term at UNC.
- Fall '08