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Unformatted text preview: carded. This completed step 2b of the algorithm. To check the remaining sequences for whether their paths passed through all the intermediary cities, I took advantage of WatsonCrick annealing in a procedure called afﬁnity separation. This process uses multiple copies of a DNA “probe” molecule that encodes the complementary name of a particular city (for example, Boston). These probes are attached to microscopic iron balls, each approximately one micron in diameter. I suspended the balls in the tube containing the remaining molecules under conditions that encouraged WatsonCrick pairing. Only those molecules that contained the desired city’s name (Boston) would anneal to the probes. Then I placed a magnet against the wall of the
Computing with DNA Copyright 1998 Scientific American, Inc. C test tube to attract and hold the metal balls to the side while I poured out the liquid phase containing molecules that did not have the desired city’s name. I then added new solvent and removed the magnet in order to resuspend the balls. Raising...
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This note was uploaded on 11/28/2011 for the course COMP 790 taught by Professor Staff during the Fall '08 term at UNC.
- Fall '08