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Unformatted text preview: IOP P UBLISHING N ANOTECHNOLOGY Nanotechnology 18 (2007) 125305 (4pp) doi:10.1088/0957-4484/18/12/125305 Scalable, low-cost, hierarchical assembly of programmable DNA nanostructures Constantin Pistol 1 and Chris Dwyer 2 1 Department of Computer Science, Duke University, Durham, NC 27708, USA 2 Department of Electrical and Computer Engineering, Duke University, Durham, NC 27708, USA Received 30 November 2006, in final form 25 January 2007 Published 23 February 2007 Online at stacks.iop.org/Nano/18/125305 Abstract We demonstrate a method for the assembly of fully programmable, large molecular weight DNA complexes. The method leverages sticky-end re-use in a hierarchical fashion to reduce the cost of fabrication by building larger complexes from smaller precursors. We have explored the use of controlled non-specific and specific binding between sticky-ends and demonstrate their use in hierarchical assembly. We conclude that it is feasible to scale this method beyond our demonstration of a fully programmable 8960 kD molecular weight 8 × 8 DNA grid for potential application to complex nanoscale system fabrication. S Supplementary data are available from stacks.iop.org/Nano/18/125305 (Some figures in this article are in colour only in the electronic version) The application of nanoscale phenomena in photonic and electronic devices is widely considered to be an important development for the future of computer systems. The material limitations of silicon and photolithography that have begun to curtail the historically steady advance of solid-state device performance are making this an increasingly important topic to industry and researchers [ 1 ]. However, few methods exist that can organize nanoscale and molecular components with the control and degree of asymmetry required to yield usefully complex circuit topologies in a scalable and low-cost manner 3 . DNA and RNA have gained popularity as a material system for creating complex, aperiodic nanostructures due to the ease with which these materials can be synthesized and controlled [ 2–7 ]. The pioneering development of the DNA crossover enables the rationale design and synthesis of structurally rigid molecular complexes from DNA [ 8–13 ]. Such methods rely on the programmability of oligonucleotide interactions and leverage the control that complementary nucleotide sequences exert over the thermodynamics of the assembly process. Recent advances in this field have produced many examples of periodic planar DNA lattice [ 12 , 14–18 ]. However, to form aperiodic 2D structures these methods require the number of unique DNA sequences 3 We consider an approach to be scalable if it can assemble aperiodic structures beyond the size limitations imposed by the finite sequence space of the sticky-ends used during the assembly....
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This note was uploaded on 11/28/2011 for the course COMP 790 taught by Professor Staff during the Fall '08 term at UNC.

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