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230_030711 - Biol 230 March 7 2011 Agenda iClicker...

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Biol 230, March 7, 2011 Agenda iClicker questions Gene regulation concepts Gene regulation in eukaryotes
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In prokaryotic genomes groups of functionally related genes along with their promoters and operators are found together in __________ A. a repressor B. an operon C. a regulatory gene D. an enhancer E. a transcriptulon
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In prokaryotic genomes groups of functionally related genes along with their promoters and operators are found together in __________ A. a repressor B. an operon C. a regulatory gene D. an enhancer E. a transcriptulon
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A mutation in the gene encoding the Trp repressor will produce a cell that: A. cannot transcribe the trp operon B. cannot translate the trp operon C. transcribes the trp operon when tryptophan is abundant but not when tryptophan is scarce D. transcribes the trp operon when tryptophan is scarce but not when tryptophan is abundant E. transcribes the trp operon when tryptophan is abundant and when tryptophan is scarce
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A mutation in the gene encoding the Trp repressor will produce a cell that: A. cannot transcribe the trp operon B. cannot translate the trp operon C. transcribes the trp operon when tryptophan is abundant but not when tryptophan is scarce D. transcribes the trp operon when tryptophan is scarce but not when tryptophan is abundant E. transcribes the trp operon when tryptophan is abundant and when tryptophan is scarce
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Default: Furnace is on on Sensor (thermometer) monitors heat output (temperature) Temp above “set” level Furnace switched off off Temp below “set” level Furnace stays on stays on The Heating Thermostat The Heating Thermostat
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Default: Gene is expressed Gene is on on Sensor ( repressor protein repressor protein ) monitors gene output (level of product of gene expression) Gene expressed above “set” level Gene expression switched off switched off Gene expressed below “set” level Gene expression stays on stays on Gene Regulation 1 Gene Regulation 1
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Default: Gene is expressed Gene is on on Sensor ( repressor repressor ) monitors gene output (ligand generated by gene expression) Gene expressed at or above “set” level Gene expression switched off switched off Gene expressed below “set” level Gene expression stays on stays on Gene Regulation 1 Gene Regulation 1 + promoter Repressor alone cannot bind operator Gene is transcribed Repressor + ligand binds operator and blocks promoter gene is switched off operator RNAP promoter operator repressor repressor ligand RNAP RNAP RNAP RNAP
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Default: Gene is expressed Gene is on on Sensor ( repressor repressor ) monitors gene output (level of gene expression) Gene expressed at or above “set” level Gene expression switched off switched off Gene expressed below “set” level Gene expression stays on stays on Regulation of the Trp Operon Regulation of the Trp Operon Repressor + ligand can bind to operator Gene turned off Repressor alone cannot bind to operator Gene stays on The Tryptophan Operon tryptophan Repressor + ligand can bind to operator Operon turned off
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Default: Gene is expressed Gene is on**
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