Lecture_12-Hb_and_O2_Binding

Lecture_12-Hb_and_O2_Binding - PROTEIN FUNCTION I Ligand...

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Unformatted text preview: PROTEIN FUNCTION I Ligand Binding-Hemoglobin non-cooperative suggests one mode of binding and release if binding is tight in lungs, release in tissues is weak if release is easy in the tissues, binding is weak in the lungs Hb and O 2 Binding ligand binding here affects binding here and here and here ` activating modulator binding the ligand at one site makes it easier to bind ligands at other sites. COOPERATIVE BINDING: THE MATH P + L PL 1.0 0.5 0.0 [L] [L] A new chemical model has to be developed P +n L PL n 1.0 0.5 0.0 [L] Hb + 4O 2 Hb(O 2 ) 4 for O 2 binding by Hb Hill Equation P +n L PL n K a = PL n [ ] P [ ] L [ ] n = L [ ] n L [ ] n + K d 1 = L [ ] n K d log 1 = n log L [ ] log K d log 1 = n log pO 2 n log P 50 n y = mx + b THE HILL PLOT log 1 = n log pO 2 n log P 50 n n would be the slope number of binding sites n H is the Hill coefFcient...
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This note was uploaded on 11/28/2011 for the course CHEM 340 taught by Professor T.baird during the Fall '11 term at S.F. State.

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Lecture_12-Hb_and_O2_Binding - PROTEIN FUNCTION I Ligand...

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