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Lecture_12-Hb_and_O2_Binding

Lecture_12-Hb_and_O2_Binding - Hb and O2 Binding...

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PROTEIN FUNCTION I Ligand Binding-Hemoglobin non-cooperative suggests one mode of binding and release if binding is tight in lungs, release in tissues is weak if release is easy in the tissues, binding is weak in the lungs Hb and O 2 Binding ligand binding here affects binding here and here and here ` activating modulator ± binding the ligand at one site makes it easier to bind ligands at other sites.
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COOPERATIVE BINDING: THE MATH P + L PL θ 1.0 0.5 0.0 [L] [L] ± A new chemical model has to be developed P + n L PL n θ 1.0 0.5 0.0 [L] Hb + 4O 2 Hb(O 2 ) 4 for O 2 binding by Hb Hill Equation P + n L PL n K a = PL n [ ] P [ ] L [ ] n θ = L [ ] n L [ ] n + K d θ 1 θ = L [ ] n K d log θ 1 θ = n log L [ ] log K d log θ 1 θ = n log pO 2 n log P 50 n y = mx + b THE HILL PLOT log θ 1 θ = n log pO 2 n log P 50 n n would be the slope number of binding sites n H is the Hill coefficient a measure of the degree of cooperativity PL n P + nL
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THE HILL PLOT log θ 1
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