Lecture_17-Michaelis-Menten_Equation

Lecture_17-Michaelis-Menten_Equation - Why study enzyme...

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ENZYME KINETICS The Michaelis-Menten Equation Why study enzyme kinetics? Determination of Catalytic Mechanisms ± the specifc steps undertaken to convert the substrate to product. His-96 His-94 His-119 O H Thr-199 O- H His-96 His-94 His-119 O H Thr-199 O- H O C O His-96 His-94 His-119 O H Thr-199 O H O - C O H O H His-96 His-94 His-119 O H Thr-199 O H O - C O H O H His-96 His-94 His-119 O H Thr-199 H O - H + O H O - C O This is the currently accepted mechanism For carbonic anhydrase Why study enzyme kinetics? Determination of Catalytic Mechanisms ± the specifc steps undertaken to convert the substrate to product. Determination of Inhibition Mechanisms ± how a molecule slows down the S P conversion by the enzyme. E I S How are the specifc steps determined? Alter the conditions of the assay and see how rates are affected. Things we can change easily: [S] total [E] total [I] total
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How does the [S] affect the rate? Time
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This note was uploaded on 11/28/2011 for the course CHEM 340 taught by Professor T.baird during the Fall '11 term at S.F. State.

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Lecture_17-Michaelis-Menten_Equation - Why study enzyme...

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