This preview shows page 1. Sign up to view the full content.
Unformatted text preview: pf regulation of digestion : hormones of gastro intestinal tract. Practical work: 1. Quantitative definition of urea in the serum of blood. 3.Qualitative opening of the urea in the urine. Pippette (ml) Sample Standard Blank Serum Reagent 5 Solution of enzyme in buffer 0,02-0,50-0,02 0.50--0,50 Mix and let stand for 10 min at 37 C Solution of nitroprusside Solution of hypochlorite 2,0 2,0 2,0 2,0 2,0 2,0 Mix immediately after addition of hypochlorite solution, let stand for 15 (26 ) and rad absorbances of a sample (1) and standard (A2) against blank 1 Fs urea (mmo/l ) = 15_____________ 2 Feference values (fs) urea mmol/l 2,61-8,35 Due urea mmol/l/ 330-590 Work 2 Opening of urea in the urine. Course of work: pour in tube 10 drops of urine, add 2,3 drops of 10% NaOH and carefully boil, then put in tube the litmus paper (red colour) then colour of litmus paper is changed from red colour to blue and is the smell of ammonia....
View Full Document
This note was uploaded on 11/29/2011 for the course RWR fref taught by Professor Erf during the Spring '11 term at Audencia Nantes Ecole de Management.
- Spring '11