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The methodical instruction for lesson 16

The methodical instruction for lesson 16 - pf regulation of...

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The methodical instruction for lesson № 16 By biochemistry for students 2 course by specialty "General medicine " from 2008 - 2009 year. The theme: METABOLISM OF PROTEINS AND AMINOACIDS . The main questions of the theme 1. The general schema of sources and pathways of transformation of amino acids in an organism of the man. 2. A transamination of amino acids. 3. Transaminases in diagnostics of deseases, vitamin B6. A deamination of amino acids, oxidative deamination. Direct and indirect deamination. Biological meaning of amino acid deamintion. 4. Decarboxylation of amino acids. 5. Formation of ammonia in the organism. Pathways of ammonia detoxification: Reductive amination, formation of asparagines and glutamine. Urea synthesis, errors of urea synthesis and excretion. 6. Hyperammoniemia, it's consequences. Practical work: 1. Quantitative definition of urea in the serum of blood. 3.Qualitative opening of the urea in the urine. Questions for independent work 1. Biochemical mechanisms
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Unformatted text preview: pf regulation of digestion : hormones of gastro – intestinal tract. Practical work: 1. Quantitative definition of urea in the serum of blood. 3.Qualitative opening of the urea in the urine. Pippette (ml) Sample Standard Blank Serum Reagent 5 Solution of enzyme in buffer 0,02-0,50-0,02 0.50--0,50 Mix and let stand for 10 min at 37 C Solution of nitroprusside Solution of hypochlorite 2,0 2,0 2,0 2,0 2,0 2,0 Mix immediately after addition of hypochlorite solution, let stand for 15 мин (26 С) and rad absorbances of a sample (А1) and standard (A2) against blank А1 Fs urea (mmo/l ) = 15_____________ А2 Feference values (fs) urea mmol/l – 2,61-8,35 Due urea mmol/l/л 330-590 Work 2 Opening of urea in the urine. Course of work: pour in tube 10 drops of urine, add 2,3 drops of 10% NaOH and carefully boil, then put in tube the litmus paper (red colour) then colour of litmus paper is changed from red colour to blue and is the smell of ammonia....
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