The methodical instruction for lesson 26

The methodical instruction for lesson 26 - 0,50 0,50 Stir...

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The methodical instruction for lesson № 26 By biochemistry for students 2 course by specialty "General medicine " from September, 15 till September, 19th 2008 - 2009 year. The theme: : Laboratory examination on a theme « Definition of the basic biochemical constant of blood and spinal fluid. » Practical work 1. Definition in serum of blood 2. Work 2.Quantitative definition of Ca in the cerebral fluid. Pipette ml sample Control solution standart Serum Dist/ water Solution 3 Reagent 3 0,50 1,00 - 050 - 1,50 - 0,50 - 1,00 0,50 0,50 Stir and into another test tubes pipette Supernatant Reagent 4 Reagent 5 1,00 0,50 0,50 1,00 0,50 0,50 1,00
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Unformatted text preview: 0,50 0,50 Stir and after exactly 20 min read the absorbances of sample A1 and standard A2 against control solution Calculation А1 177 х __________ Creatinine мkмоl/l = А2 Reference values: мkмоl/l Men -1-115 Women- 53-97 . Work 2 Quantitative definition of Ca in the cerebral fluid. Procedure: In flask pour in 5 ml solution of murexid, add 0,2 ml of the cerebral fluid is formed the pink color and that trilon - B till will be pink-blue color . Count: Concentration /Ca=X/ trilon B * 0,12*100/ Normal Ca = 1,22 -1,67 mm/l / in the cerebral fluid/. Result: Conclusion:...
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