Lecture15 - could be a better fluorescent tag for biology...

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MCDB321 Introductory Plant Physiology Mar 10, 2011 Phototropism and Blue-Light Receptors
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A sensing mechanism that depended on light gradient leads to differential cell elongation
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Action Spectrum of Blue-Light Induced Phototropism
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An Arabidopsis long-hypocotyl mutant (hy4/cry1) is defective An Arabidopsis long-hypocotyl mutant (hy4/cry1) is defective in a blue-light receptor involved in regulating stem elongation in a blue-light receptor involved in regulating stem elongation
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The HY4 gene encodes a 75 kDa protein with significant sequence identity to microbial DNA photolyase, a blue-light activated DNA repair enzyme It was renamed Cryptochrome 1 (CRY1) When expressed in E. coli , CRY1 binds FAD and a pterin, but lacks detectable photolyase activity
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Screening for non-phototropical mutants in Arabidopsis led to the isolation of nph1 mutant
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Absorption spectrum Blue-light irradiation causes a conformational changes in Phot1, triggering autophosphorylation and starting the blue-light signal transduction
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The LOV domain
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Unformatted text preview: could be a better fluorescent tag for biology studies Chloroplasts relocate their positions in a cell in response to the intensity of incident blue-light, moving to the side wall of the cell to avoid strong light, but gathering at the front face under weak light to maximize light interception. When the CAV1 gene was cloned, it was found that it encodes PHOT2. phot2 can’t survive under strong light Stomatal opening is also a blue-light-regulated process Blue-light activates a proton-pumping H +-ATPase in the guard cell plasma membrane K + in channel is activated, accompanied by influxes of Cl-and malate 2- (to balance the accumulation of K + inside guard cells), resulting in an increase in osmotic potential Both Phot1 and Phot2 are Blue-light Receptors Regulating Stomatal Opening Weak light Phototropism Strong light Chloroplast movement PHOT1 and PHOT2 function redundantly Stomatal opening Chloroplast movement under both strong and weak blue-light...
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This note was uploaded on 11/30/2011 for the course BIOLOGY 321 taught by Professor Min during the Winter '11 term at University of Michigan.

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Lecture15 - could be a better fluorescent tag for biology...

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