Review Questions for Lecture 191.Explain how auxin was discovered. Describe a simple biological assay for quantifying auxin.2.What are the structural similarities of the compounds that have auxin activity? What is theprinciple auxin in higher plants? What is the relationship between auxin and the AgentOrange used widely during the Vietnam War?3.Where is auxin synthesized in plants? How can plants regulate the cellular concentration offree auxin? What method can you use to locate the organ/tissue/cells that accumulate auxin(what reporter gene can you use to tell if the tissue accumulates auxin?)4.Why did people think that auxin is made from tryptophan, an essential amino acid? Whatexperiments led to the discovery of a tryptophan-independent pathway of auxinbiosynthesis?5.How can you demonstrate that auxin transport is unidirectional? Describe the chemiosmoticmodel for a polar auxin transport. Explain why the presence of an efflux auxin carrier and itslocalization at the basal end of a plant cell are essential for the polar transport of auxin.
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